In vivo and in vitro anti-inflammatory activity of red clover Trifolium pratense dry extract

作者：Ramos Graziele P; Apel Miriam A; de Morais Claudia B; Ceolato Paula C; Schapoval Elfrides E S; Dall'Agnol Miguel; Zuanazzi Jose A S^*

来源：Brazilian Journal of Pharmacognosy, 2012, 22(1): 176-180.

DOI：10.1590/S0102-695X2011005000200

• Trifolium pratense
• isoflavones
• anti-inflammatory
• chemotaxis
• leukocyte migration
• rat paw edema

Red clover Trifolium pratense L., Fabaceae, contains four isoflavones, mainly formononetin and biochanin A, and in smaller concentrations, daidzein and genistein. These compounds have gained a lot interest due to its human health benefits, such as estrogenic and progestogenic activities, antioxidant, anti-cancer and others. The objective of this study was to determine in vivo and in vitro anti-inflammatory activity of red clover dry extract. The in vitro anti-inflammatory activity was assayed by the technique using the Boyden chamber method, evaluating the leukocyte migration inhibition (chemotaxis). The in vivo anti-inflammatory activity was tested by a carrageenan-induced rat paw edema test. The results of anti-inflammatory in vitro test showed that there was a significant inhibition of leukocyte migration at the concentrations of 100, 50, 25, 10 and 5 mu g/mL of red clover dry extract, these doses resulted in 94.73, 95.39, 94.73, 84.68 and 78.75% of inhibition for each dose, respectively. The anti-inflammatory in vivo test resulted in a significant activity in both tested doses (100 and 50 mg/kg of red clover dry extract) and at each tested time. The average percentage of edema inhibition was 63.37%. The findings of this study suggested that red clover extract might be suitable for the treatment of inflammatory diseases.

• 出版日期2012-2