Deglycosylation of Shaker K-v channels affects voltage sensing and the open closed transition

作者：Lopez Rodriguez Angelica^*; Holmgren Miguel^*

来源：Journal of General Physiology, 2018, 150(7): 1025-1034.

DOI：10.1085/jgp.201711958

Most membrane proteins are subject to posttranslational glycosylation, which influences protein function, folding, solubility, stability, and trafficking. This modification has been proposed to protect proteins from proteolysis and modify protein-protein interactions. Voltage-activated ion channels are heavily glycosylated, which can result in up to 30% of the mature molecular mass being contributed by gtycans. Normally, the functional consequences of glycosylation are assessed by comparing the function of fully glycosylated proteins with those in which glycosylation sites have been mutated or by expressing proteins in model cells lacking glycosylation enzymes. Here, we study the functional consequences of deglycosylation by PNGase F within the same population of voltage-activated potassium (K-v) channels. We find that removal of sugar moieties has a small, but direct, influence on the voltage-sensing properties and final opening-closing transition of Shaker K-v channels. Yet, we observe that the interactions of various ligands with different domains of the protein are not affected by deglycosylation. These results imply that the sugar mass attached to the voltage sensor neither represents a cargo for the dynamics of this domain nor imposes obstacles to the access of interacting molecules.

• 出版日期2018-7