Autophagy controls centrosome number by degrading Cep63

作者：Watanabe Yuichiro; Honda Shinya; Konishi Akimitsu; Arakawa Satoko; Murohashi Michiko; Yamaguchi Hirofumi; Torii Satoru; Tanabe Minoru; Tanaka Shinji; Warabi Eiji; Shimizu Shigeomi^*

来源：Nature Communications, 2016, 7(1): 13508.

DOI：10.1038/ncomms13508

Centrosome number is associated with the chromosome segregation and genomic stability. The ubiquitin-proteasome system is considered to be the main regulator of centrosome number. However, here we show that autophagy also regulates the number of centrosomes. Autophagy-deficient cells carry extra centrosomes. The autophagic regulation of centrosome number is dependent on a centrosomal protein of 63 (Cep63) given that cells lacking autophagy contain multiple Cep63 dots that are engulfed and digested by autophagy in wild-type cells, and that the upregulation of Cep63 increases centrosome number. Cep63 is recruited to autophagosomes via interaction with p62, a molecule crucial for selective autophagy. In vivo, hematopoietic cells from autophagy-deficient and p62(-/-) mice also contained multiple centrosomes. These results indicate that autophagy controls centrosome number by degrading Cep63.

• 出版日期2016-11-21