Altered immune response of immature dendritic cells following dengue virus infection in the presence of specific antibodies

作者：Torres Silvia; Flipse Jacky; Upasani Vinit C; van der Ende Metselaar Heidi; Urcuqui Inchima Silvio; Smit Jolanda M; Rodenhuis Zybert Izabela A^*

来源：Journal of General Virology, 2016, 97(7): 1584-1591.

DOI：10.1099/jgv.0.000491

Dengue virus (DENV) replication is known to prevent maturation of infected dendritic cells (DCs) thereby impeding the development of adequate immunity. During secondary DENV infection, dengue-specific antibodies can suppress DENV replication in immature DCs (immDCs), however how dengue-antibody complexes (DENV-IC) influence the phenotype of DCs remains elusive. Here, we evaluated the maturation state and cytokine profile of immDCs exposed to DENV-ICs. Indeed, DENV infection of immDCs in the absence of antibodies was hallmarked by blunted upregulation of CD83, CD86 and the major histocompatibility complex molecule HLA-DR. In contrast, DENV infection in the presence of neutralizing antibodies triggered full DC maturation and induced a balanced inflammatory cytokine response. Moreover, DENV infection under non-neutralizing conditions prompted upregulation of CD83 and CD86 but not HLA-DR, and triggered production of pro inflammatory cytokines. The effect of DENV-IC was found to be dependent on the engagement of FcyRIla. Altogether, our data show that the presence of DENV-IC alters the phenotype and cytokine profile of DCs.

• 出版日期2016-7