Detection of Treponema pallidum in Aqueous Humor by Real-time Polymerase Chain Reaction

作者：Cornut Pierre Loic^*; Sobas Chantal Roure; Perard Laurent; De Bats Flore; Salord Helene; Manificat Helene Janin; Denis Philippe; Burillon Carole

来源：Ocular Immunology and Inflammation, 2011, 19(2): 127-128.

DOI：10.3109/09273948.2010.531175

• aqueous humor
• ocular syphilis
• real-time PCR
• Treponema pallidum
• uveitis

Methods: A real-time (RT) PCR assay targeting the polymerase 1 gene of Treponema pallidum was performed in aqueous humor samples collected in consecutive patients with ocular syphilis.
Results: Five patients presenting with optic neuritis (1), posterior placoid chorioretinitis (1), or panuveitis with retinitis (3) were tested. The PCR results were positive in the 3 cases of panuveitis and negative in the 2 other cases.
Conclusion: RT-PCR for Treponema pallidum in aqueous humor is useful to confirm syphilitic panuveitis.

• 出版日期2011-4