A combined approach of vesicle formulations and microneedle arrays for transcutaneous immunization against hepatitis B virus

作者：Hirschberg Hoang; van Kuijk Sandra; Loch Jessica; Jiskoot Wim; Bouwstra Joke; Kersten Gideon^*; Amorij Jean Pierre

来源：European Journal of Pharmaceutical Sciences, 2012, 46(1-2): 1-7.

DOI：10.1016/j.ejps.2012.01.013

• Microneedles
• Elastic vesicles
• Transcutaneous immunization
• Hepatits B vaccine

In the search for an optimal approach for the transcutaneous immunization (TCI) of hepatitis B surface antigen (HBsAg), two vesicle formulations, L595 vesicles (composed of sucrose-laurate ester and octaoxyethylene-laurate ester) and sPC vesicles (composed of soybean phosphatidylcholine and Span-80) were prepared and characterized in vitro and in vivo. HBsAg was associated to the vesicles, resulting in sPC-HBsAg vesicles (+/- 170 nm) with 79% HBsAg association and L595-HBsAg vesicles (+/- 75 nm) with only 29% HBsAg association.
The vesicles induced in mice via TCI an antibody response only when the skin was pretreated with microneedles. This response was improved by the adjuvant cholera toxin. The sPC HBsAg vesicle formulations showed to be the most immunogenic for TCI, which was related to the higher degree of HBsAg association.

• 出版日期2012-5-12