Antihepatic Fibrosis Effect of Active Components Isolated from Green Asparagus (Asparagus officinalis L.) Involves the Inactivation of Hepatic Stellate Cells

作者：Zhong, Chunge; Jiang, Chunyu; Xia, Xichun; Mu, Teng; Wei, Lige; Lou, Yuntian; Zhang, Xiaoshu; Zhao, Yuqing; Bi, Xiuli^*

来源：Journal of Agricultural and Food Chemistry, 2015, 63(26): 6027-6034.

DOI：10.1021/acs.jafc.5b01490

• Asparagus officinalis L
• active components
• antihepatic fibrosis
• TGF-beta 1/Smad signaling
• c-Jun N-terminal kinases
• ERK

Green asparagus (Asparagus officinalis L.) is a vegetable with numerous nutritional properties. In the current study, a total of 23 compounds were isolated from green asparagus, and 9 of these compounds were obtained from this genus for the first time. Preliminary data showed that the ethyl acetate (EtOAc)-extracted fraction of green asparagus exerted a stronger inhibitory effect on the growth of t-HSC/Cl-6 cells, giving an IC50 value of 45.52 mu g/mL. The biological activities of the different compounds isolated from the EtOAc-extracted fraction with respect to antihepatic fibrosis were investigated further. Four compounds, C3, C4, C10, and C12, exhibited profound inhibitory effect on the activation of t-HSC/Cl-6 cells induced by TNF-alpha. The activation t-HSC/Cl-6 cells, which led to the production of fibrotic matrix (TGF-beta 1, activin C) and accumulation of TNF-alpha, was dramatically decreased by these compounds. The mechanisms by which these compounds inhibited the activation of hepatic stellate cells appeared to be associated with the inactivation of TGF-beta 1/Smad signaling and c-Jun N-terminal kinases, as well as the ERK phosphorylation cascade.

• 出版日期2015-7-8
• 单位辽宁大学; 沈阳药科大学