A Sensitive SERS Quantitative Analysis Method for Amino Acids Using Ruhemann's Purple as Molecular Probe in Triangle Nanosilver Sol Substrate

作者：Luo, Yanghe; Wen, Guiqing; Ma, Lu; Liang, Aihui^*; Jiang, Zhiliang^*

来源：Plasmonics, 2017, 12(2): 299-308.

DOI：10.1007/s11468-016-0264-8

• Amino acid
• Ninhydrin
• Ruhemann's purple
• AgNT
• SERS

The stable silver nanotriangle (AgNT) sol was prepared by reduction of silver ions with sodium borohydride in the presence of H2O2 and sodium citrate. Under the action of NaCl, AgNTs were aggregated to a highly surface-enhanced Raman scattering (SERS) active substrate. In pH 6.0 Na2HPO4-NaH2PO4 buffer solution (PBS) at 80 A degrees C water bath, ninhydrin reacted with amino acids to form blue-violet complex Ruhemann's purple (RP), and the RP molecules adsorbed on the aggregated AgNT surfaces that exhibited a strong SERS peak at 657 cm(-1). The peak was linear to amino acid concentration in the range of 0.7-99.8 mu mol/L, with a detection limit (DL) of 0.5 mu mol/L. This SERS method was applied to detect amino acid in samples, with satisfactory results. In addition, the analytical system was also investigated by resonance Rayleigh scattering (RRS), absorption, and electron microscope techniques.

• 出版日期2017-4
• 单位贺州学院; 广西师范大学