Monitoring the rumen pectinolytic bacteria Treponema saccharophilum using real-time PCR

作者：Liu, Jing; Wang, Jia-Kun; Zhu, Wen; Pu, Yi-Yi; Guan, Le-Luo; Liu, Jian-Xin^*

来源：FEMS Microbiology Ecology, 2014, 87(3): 576-585.

DOI：10.1111/1574-6941.12246

• pectin
• forage
• 16S rRNA gene
• Treponema saccharophilum

Treponema saccharophilum is a pectinolytic bacterium isolated from the bovine rumen. The abundance of this bacterium has not been well determined, reflecting the lack of a reliable and accurate detection method. To develop a rapid method for monitoring T.saccharophilum, we performed pyrosequencing of genomic DNA isolated from rumen microbiota to explore the 16S rRNA gene sequences of T.saccharophilum candidates. Species-specific primers were designed based on fifteen sequences of partial 16S rRNA genes generated through pyrosequencing with 97% or higher similarity with T.saccharophilum DSM2985 along with sequence from type strain. The relative abundance of T.saccharophilum was quantified in both in vitro and in vivo rumen systems with varied pectin-containing forages using real-time PCR. There was a clear association of T.saccharophilum with alfalfa hay, which contains more pectin than Chinese wild rye hay or corn stover. The relative abundance of T.saccharophilum was as high as 0.58% in vivo, comparable with the population density of other common rumen bacteria. It is recognized that T.saccharophilum plays an important role in pectin digestion in the rumen.

• 出版日期2014-3
• 单位浙江大学