A practical kit for micro-scale application of the ceiling crystallisation method

作者:Adawy Alaa*; Corbeek Wil; de Ronde Erik; van Enckevort Willem J P; de Grip Willem J; Vlieg Elias
来源:CrystEngComm, 2015, 17(13): 2602-2605.
DOI:10.1039/c4ce01814a

摘要

We designed a kit to facilitate the optimisation of protein crystal growth by means of the ceiling crystallisation method. This ceiling kit allows for diffusion-limited crystal growth with a total volume down to 10 mu l per trial and air-tight sealing for any type of protein sample. The ceiling crystals grow to sufficient size for diffraction experiments. In this highlight, we fully describe the features of this kit and how easily it can be used to yield protein crystals.

  • 出版日期2015