Assessing the usefulness of clostridia spores for evaluating sewage sludge hygienization

作者：Martin Diaz Julia; Ruiz Hernando Maria; Astals Sergi; Lucena Francisco

来源：Bioresource Technology, 2017, 225: 286-292.

DOI：10.1016/j.biortech.2016.11.075

• Clostridium spores
• Anaerobic digestion
• Sewage sludge
• Hygienization treatment
• Indicator

The capability of clostridia spores to act as pathogen indicators in sewage sludge treatment was investigated. Sulfite-reducing clostridia and E. coli levels were monitored during waste activated sludge pre-treatments (alkali and ultrasound) and its subsequent mesophilic anaerobic digestion. E. coli was maintained or reduced depending on treatment type and intensity. However, alkali pre-treatment (35.3 gNaOH/kg TS) by itself and alkali (157 gNaOH/kg TS) and ultrasound (27,000 kJ/kg TS) pre-treatments followed by anaerobic digestion provoked reproducible clostridia increases. Specifically, up to 2.7 log(10) after 35.3 gNaOH/kg TS pre-treatment and up to 1.9 and 1.1 log(10) after digesting the 157 gNaOH/kg TS and 27,000 kJ/kg TS pre-treated sludge, respectively. Having rejected the hypotheses of sporulation and floc dissipation, the most plausible explanation for these clostridia increases is re-growth. These results question the suitability of clostridia spores as indicators of sludge treatment and other biological treatments where clostridia may have a role.

• 出版日期2017-2