Anisotropic light scattering of individual sickle red blood cells

作者：Kim Youngchan; Higgins John M; Dasari Ramachandra R; Suresh Subra; Park YongKeun^*

来源：Journal of Biomedical Optics, 2012, 17(4): 040501.

DOI：10.1117/1.JBO.17.4.040501

• red blood cell
• sickle cell disease
• light scattering
• quantitative phase microscopy

We present the anisotropic light scattering of individual red blood cells (RBCs) from a patient with sickle cell disease (SCD). To measure light scattering spectra along two independent axes of elongated-shaped sickle RBCs with arbitrary orientation, we introduce the anisotropic Fourier transform light scattering (aFTLS) technique and measured both the static and dynamic anisotropic light scattering. We observed strong anisotropy in light scattering patterns of elongated-shaped sickle RBCs along its major axes using static aFTLS. Dynamic aFTLS analysis reveals the significantly altered biophysical properties in individual sickle RBCs. These results provide evidence that effective viscosity and elasticity of sickle RBCs are significantly different from those of the healthy RBCs.

• 出版日期2012-4