Molecular mapping of the genomic region conferring resistance to soybean stem canker in Hutcheson soybean

作者：Chiesa M A; Cambursano M V; Pioli R N; Morandi E N

来源：Molecular Breeding, 2017, 37(5): 65.

DOI：10.1007/s11032-017-0660-6

• Diaporthe phaseolorum var. meridionalis
• Disease resistance genes
• Amplified fragment length polymorphism(AFLP)
• Simple sequence repeat (SSR)
• Bulk segregant analysis (BSA)
• Linkage analysis
• Marker-assisted breeding

Genetic resistance to soybean stem canker, caused by the fungus Diaporthe phaseolorum var. meridionalis (Dpm), is controlled by five major, dominant, nonallelic genes Rdm1 to Rdm5. A genomic region containing the Rdm4 and Rdm5 genes was first described in Hutcheson soybean, where they were found to confer specific resistance to Argentinean physiological races of Dpm. Here, we report the genetic mapping of Rdm4 and Rdm5 loci using two pheno- and genotypically characterized F-2:3 populations derived from Hutcheson cultivar. The mapping populations were screened with amplified fragment length polymorphism (AFLP) markers using bulk segregant analysis, and with simple sequence repeat (SSR) markers. Linkage analysis indicated that the Rdm4 and Rdm5 resistance loci were located in a genomic region collinear with the molecular linkage group (MLG) A2 (chromosome 8) of the soybean genetic map. The linkage group contains two SSR markers, Sat_162 and Satt233, flanking the Rdm4 and Rdm5 loci. These SSR will be useful to increase the efficiency of selection in breeding programs aimed to incorporate Rdm4 and Rdm5 genes into soybean elite germplasm.

• 出版日期2017-5