Granulocyte-macrophage colony-stimulating factor, a potent adjuvant for polarization to Th-17 pattern: an experience on HIV-1 vaccine model

作者：Mahdavi Mehdi^*; Tajik Amir Hossein; Ebtekar Massoumeh; Rahimi Roghieh; Adibzadeh Mohammad Mehdi; Moozarmpour Hamid Reza; Beikverdi Mohammad Sadegh; Olfat Soophie; Hassan Zuhair Mohammad; Choopani Mohammad; Kameli Morteza; Hartoonian Christine

来源：Acta Pathologica, Microbiologica et Immunologica Scandinavica, 2017, 125(6): 596-603.

DOI：10.1111/apm.12660

• Granulocyte-macrophage colony-stimulating factor
• adjuvant
• Th-17
• HIV-1 DNA vaccine
• immune response

Cytokines are mediators for polarization of immune response in vaccines. Studies show that co-immunization of DNA vaccines with granulocyte-macrophage colony-stimulating factor (GM-CSF) can increase immune responses. Here, experimental mice were immunized with HIV-1(tat/pol/gag/env) DNA vaccine with GM-CSF and boosted with recombinant vaccine. Lymphocyte proliferation with Brdu and CTL activity, IL-4, IFN-gamma, IL-17 cytokines, total antibody, and IgG1 and IgG2a isotypes were assessed with ELISA. Results show that GM-CSF as adjuvant in DNA immunization significantly increased lymphocyte proliferation and IFN-gamma cytokines, but CTL response was tiny increased. Also GM-CSF as adjuvant decreased IL-4 cytokine vs mere vaccine group. IL-17 in the group that immunized with mixture of DNA vaccine/GMCSF was significantly increased vs DNA vaccine group. Result of total antibody shows that GM-CSF increased antibody response in which both IgG1 and IgG2a increased. Overall, results confirmed the beneficial effect of GM-CSF as adjuvant to increase vaccine immunogenicity. The hallmark result of this study was to increase IL-17 cytokine with DNA vaccine/GM-CSF immunized group. This study for the first time provides the evidence of the potency of GM-CSF in the induction of IL-17 in response to a vaccine, which is important for control of infection such as HIV-1.

• 出版日期2017-6