Autonomous Metabolic Oscillations Robustly Gate the Early and Late Cell Cycle

作者：Papagiannakis Alexandros; Niebel Bastian; Wit Ernst C; Heinemann Matthias^*

来源：Molecular Cell, 2017, 65(2): 285-295.

DOI：10.1016/j.molcel.2016.11.018

Eukaryotic cell division is known to be controlled by the cyclin/cyclin dependent kinase (CDK) machinery. However, eukaryotes have evolved prior to CDKs, and cells can divide in the absence of major cyclin/CDK components. We hypothesized that an autonomous metabolic oscillator provides dynamic triggers for cell-cycle initiation and progression. Using microfluidics, cell-cycle reporters, and single-cell metabolite measurements, we found that metabolism of budding yeast is a CDK-independent oscillator that oscillates across different growth conditions, both in synchrony with and also in the absence of the cell cycle. Using environmental perturbations and dynamic single-protein depletion experiments, we found that the metabolic oscillator and the cell cycle form a system of coupled oscillators, with the metabolic oscillator separately gating and maintaining synchrony with the early and late cell cycle. Establishing metabolism as a dynamic component within the cellcycle network opens new avenues for cell-cycle research and therapeutic interventions for proliferative disorders.

• 出版日期2017-1-19