A DiCre recombinase-based system for inducible expression in Leishmania major

作者：Santos Renato E R S; Silva Gabriel L A; Santos Elaine V; Duncan Samuel M; Mottram Jeremy C; Damasceno Jeziel D^*; Tosi Luiz R O^*

来源：Molecular and Biochemical Parasitology, 2017, 216: 45-48.

DOI：10.1016/j.molbiopara.2017.06.006

• DiCre recombinase
• Leishmania
• Inducible expression
• DNA damage response
• 9-1-1 complex
• Rad9-Rad1-Hus1

Here we present the establishment of an inducible system based on the dimerizable Cre recombinase (DiCre) for controlled gene expression in the protozoan parasite Leishmania. Rapamycin-induced DiCre activation promoted efficient flipping and expression of gene products in a time and dose-dependent manner. The DiCre flipping activity induced the expression of target genes from both integrated and episomal contexts broadening the applicability of the system. We validated the system by inducing the expression of both full length and truncated forms of the checkpoint protein Rad9, which revealed that the highly divergent C-terminal domain of Rad9 is necessary for proper subcellular localization. Thus, by establishing the DiCre-based inducible system we have created and validated a robust new tool for assessing gene function in Leishmania.

• 出版日期2017-9