A flow-injection chemiluminescence method for the determination of human serum albumin, using the reaction of fluorescein-human serum albumin-sodium hypochlorite by the enhancement effect of the cationic surfactant cetyltrimethylammonium bromide

作者：Huang Chun Bao^*; Zhang Kai; Liu Xue Lian; Wang Sheng Fu

来源：Luminescence, 2007, 22(5): 393-400.

DOI：10.1002/bio.975

• surfactant
• fluorescein
• sensitization
• flow-injection chemiluminescence
• human serum albumin
• density functional
• theory

The interaction between surfactant and fluorescein was studied, using a fluorescence spectroscopy and flow-injection (FI) chemiluminescence (CL) method. It was found that the cationic surfactant cetyltrimethylammoniurn bromide (CTAB) could cause the structural transformation of fluorescein from the quinone to the spirolactone form, and greatly enhance the CL intensity of the fluorescein-human serum albumin (HSA) complex. Based on this finding, a rapid and sensitive FI-CL method was developed for the determination of HSA. Under the optimum conditions, the proposed method has a linear range of 0.05-24.0 mu g/mL, with a detection limit of 0.03 mu g/mL for HSA (3 sigma). The relative standard deviation (RSD) of 1.2 mu g/mL HSA (n = 8) is 0.8%. The method was applied to the determination of protein content in urine samples, with satisfactory results. Density functional theory was used to study the mechanism of surfactant-enhanced CL intensity of the fluorescein-HSA complex.

• 出版日期2007-10
• 单位黄冈师范学院; 湖北大学