Development and characterization of EST-derived SSRs from a 'totipotent' cDNA library of durum wheat

作者：Gadaleta A^*; Mastrangelo A M; Russo M A; Giove S L; D'onofrio O; Mango T; Cellini F; Blanco A; Cattivelli L; Cifarelli R A

来源：Plant Breeding, 2010, 129(6): 715-717.

DOI：10.1111/j.1439-0523.2009.01760.x

• EST-SSR
• wheat
• microsatellites
• molecular markers
• 'totipotent' cDNA library

P>A collection of about 8000 expressed sequence tags (ESTs) deriving from a 'totipotent' cDNA library constructed in the durum wheat cv. 'Ofanto' (Patent n. WO2005003344) were screened to search for simple sequence repeat (SSR) motifs. Five hundred twenty-three SSRs were identified in 460 EST sequences with an average frequency of one SSR per 6.96 kb. Out of 523 microsatellites, 364 were successfully used to design specific primer pairs, while for the remaining ones the available sequence information did not allow the identification of suitable primers. Among the EST-derived SSRs, the trinucleotides were the most frequent (49.1%), followed by dinucleotides (26%) and mononucleotides (21.4%), while motif size of five or six nucleotides were rare, 0.6% and 0.2%, respectively. A total of 125 EST-derived SSRs chosen among di- and tri-nucleotides were further characterized in a panel of 12 tetraploid wheat accessions. The average polymorphism level of the selected SSR was equal to 49.6%. Table S1 contains all the information on primer sequences, chromosome positions and putative function.

• 出版日期2010-12