Adenylate cyclase orthologues in two filamentous entomopathogens contribute differentially to growth, conidiation, pathogenicity, and multistress responses

作者：Wang, Jie; Zhou, Gang; Ying, Sheng-Hua; Feng, Ming-Guang^*

来源：Fungal Biology, 2014, 118(4): 422-431.

DOI：10.1016/j.funbio.2014.03.001

• Asexual development
• Biocontrol potential
• Entomopathogenic fungi
• Functional differentiation

Adenylate cyclase (AC) is a core element of cAMP signalling network. Here we show functional diversity and differentiation of Beauveria bassiana AC (BbAC) and Metarhizium robertsii AC (MrAC). Severe growth defects occurred in Delta BbAC and Delta MrAC grown on nutrition-rich SDAY and several minimal media but were largely alleviated by adding cAMP to SDAY. Conidial yield increased greatly in ABbAC but decreased in Delta MrAC. During colony growth, ABbAC was highly sensitive to oxidation, high osmolarity, cell wall perturbation, carbendazim fungicide, Mn2+, Zn2+, Fe3+, and EDTA but more tolerant to Cu2+ while Delta MrAC showed higher osmotolerance, decreased sensitivity to Fe3+, and null response to carbendazim or cell wall stress despite similar responses to oxidation and other metal ions. Conidial UV-B resistance decreased by 32 % in ABbAC and 22 % in Delta MrAC despite little change in their theromotolerance. Median lethal time (LT50) estimates of ABbAC and Delta MrAC against susceptible insects were 10.9 and 1.4 d longer than those from wild-type strains respectively. All the phenotypic changes were restored to wild-type levels by each gene complementation. Taken together, BbAC and MrAC regulated differentially conidiation, pathogenicity, and multistress responses in B. bassiana and M. robertsii, thereby making different contributions to their biocontrol potential.

• 出版日期2014-4
• 单位广东省微生物研究所; 浙江大学