Altered expression of microRNAs in patients with pouchitis after restorative proctocolectomy

作者：Inoue Emi; Hata Keisuke; Kimura Hideaki; Yamaguchi Kiyoshi; Nojima Masanori; Endo Itaru; Shinozaki Masaru^*

来源：Surgery Today, 2017, 47(12): 1484-1491.

DOI：10.1007/s00595-017-1550-6

• MicroRNA
• Pouchitis
• Ulcerative colitis
• Interleukin 17 receptor A

Pouchitis is the most common long-term complication of restorative proctocolectomy with ileal pouch-anal anastomosis. We investigated alterations in the expression of microRNAs, noncoding RNAs that act as potent negative regulators of gene expression, in pouchitis.
The subjects of this study were 16 patients with diagnosed pouchitis and 48 patients without pouchitis after restorative proctocolectomy, performed for ulcerative colitis. Total RNA was extracted from biopsies and microRNAs were quantified using a real-time polymerase chain reaction.
The expression of microRNA 21 and 223 was higher, whereas that of microRNA 192 and 196a was lower, in the inflamed mucosa from the pouchitis patients than in the mucosa from the non-pouchitis patients. The levels of 14 microRNAs were significantly lower in the mucosa from the pouchitis patients, than in the non-inflamed proximal ileal mucosal samples. The expression of microRNA 192 was remarkably reduced in pouchitis. A significant negative correlation was found between microRNA 192 and interleukin 17 receptor A mRNA levels.
Significant alteration in miRNA expression in line with inflammatory bowel disease was evident in the mucosa from the pouchitis patients. Interleukin 17 receptor A may be involved in the pathogenesis of pouchitis through the downregulation of microRNA 192.

• 出版日期2017-12
• 单位河北医科大学