Multi-walled carbon nanotubes/sol-gel-derived silica/chitosan nanobiocomposite for total cholesterol sensor

作者：Solanki Pratima R^*; Kaushik Ajeet; Ansari Anees A; Tiwari A; Malhotra B D

来源：Sensors and Actuators B: Chemical , 2009, 137(2): 727-735.

DOI：10.1016/j.snb.2008.12.044

• Cholesterol biosensor
• Chitosan
• MWCNT
• Nanobiocomposite

Cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) have been immobilized via glutaraldehyde as a cross-linker onto sol-gel-derived silica (SiO(2))/chitosan (CHIT)/multi-walled carbon nanotubes (MWCNT)based nanobiocomposite film deposited onto indium-tin-oxide (ITO) glass for estimation of esterified cholesterol. The ChEt-ChOx/MWCNT/SiO(2)-CHIT/ITO bioelectrode characterized using Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and electrochemical techniques and shows response time of 10 s, linearity as 10-500 mg/dL for esterified cholesterol (cholesterol oleate), sensitivity as 3.8 mu A/mM and shelf-life of about 10 weeks under refrigerated conditions. The value of Michaelis-Menten constant (K(m)) estimated as 0.052 mM using Lineweaver-Burke plot indicates high affinity of ChEt and ChOx to cholesterol oleate. Attempts have been made to utilize this electrode for estimation of total cholesterol in blood serum samples.

• 出版日期2009-4-2