A flow injection chemiluminescence method for the determination of fluoroquinolone derivative using the reaction of luminol and hydrogen peroxide catalyzed by gold nanoparticles

作者：Wang Lun^*; Yang Ping; Li Yongxin; Chen Hongqi; Li Maoguo; Luo Fabao

来源：Talanta, 2007, 72(3): 1066-1072.

DOI：10.1016/j.talanta.2006.12.050

• chemiluminescence
• gold nanoparticles
• fluoroquinolones derivative

Based on the enhancement of chemiluminescence (CL) of luminol-hydrogen peroxide-gold nanoparticles system by fluoroquinolones (FQs), a novel and rapid CL method is reported for the determination of FQs derivatives. Under the optimum conditions, the CL intensity is proportional to the concentration of FQs derivative in solution. The corresponding linear regression equations are established over the range of 0.08-1.28 mu g/mL for norfloxacin, 0.013-1.32 mu g/mL for ciprolloxacin, 0.014-1.4 mu g/mL for lomefloxacin, 0.029-1.46 mu g/mL for fleroxacin, 0.02-1.0 mu g/mL for ofloxacin and 0.01-1.44 mu g/mL for levofloxacin, respectively. The limits of detection (S/N= 3) are 3.2, 9.5, 7.0, 9.0, 8.0, and 8.0 ng/mL with the relative standard deviation (n = 11) 4.3, 1.5, 1.9, 1.3, 1.6 and 2.1% for norfloxacin, ciprofloxacin, lomefloxacin, fleroxacin, ofloxacin and levofloxacin, respectively. This proposed method has been applied to detect FQs derivatives in human urine successfully.

• 出版日期2007-5-15
• 单位安徽师范大学