Microwave-assisted derivatisation and LC-MS/MS determination of nitrofuran metabolites in farm-raised prawns (Penaeus monodon)

作者：Palaniyappan Venkatesh^*; Nagalingam Arun Kumar; Ranganathan Hari Prasad; Kandhikuppam Krishnamoorthy Bharathi; Kothandam Hari Prasath; Vasu Soumya

来源：Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure& Risk Assessment, 2013, 30(10): 1739-1744.

DOI：10.1080/19440049.2013.816896

• LC-MS
• MS
• microwave-assisted derivatisation
• nitrofuran metabolites
• prawns

A new microwave-assisted derivatisation and LC-MS/MS method has been developed for the analysis of nitrofuran metabolites - 3-amino-5-morpholino-methyl-1,3-oxa-zolidinone (AMOZ), 3-amino-2-oxazolidinone (AOZ), 1-aminohydantoin (AHD) and semicarbazide (SEM) - in farm-raised prawns (Penaeus monodon) from the coastal regions of South India. Analysis was carried out by reverse-phase column (Phenomenex Luna C-18) with gradient elution using mobile phase A (0.02% acetic acid in water) and mobile phase B (0.02% acetic acid in acetonitrile), at a flow rate of 200lmin(-1) and an injection volume of 20l. Microwave-assisted derivatisation was achieved in 6min with good recovery. The results showed that the samples collected from Andhra Pradesh and Karnataka contained residues of nitrofuran metabolites in the range from 5.0 to 40ngg(-1). This work emphasises the importance of ensuring the safety of seafood and that a new method of derivatisation is applicable for the analysis of nitrofuran metabolites in seafood.

• 出版日期2013-10-1