microRNA-210 is involved in the regulation of postmenopausal osteoporosis through promotion of VEGF expression and osteoblast differentiation

作者：Liu, Xiao-Dong^*; Cai, Feng; Liu, Liang; Zhang, Yan; Yang, An-Li

来源：Biological Chemistry, 2015, 396(4): 339-347.

DOI：10.1515/hsz-2014-0268

• BMSC differentiation
• miR-210
• postmenopausal osteoporosis
• VEGF

MicroRNAs (miRNAs) are small non-protein-codingRNAs that function as negative gene expression regulators. miRNA-210 (miR-210) has recently been recognized in the pathogenesis of osteonecrosis associated with angiogenesis. Herein we aimed to explore the clinical significance of miR-210 treatment for postmenopausal osteoporosis. The expression of miR-210 was detected in bone marrow mesenchymal stem cells (BMSCs) in vitro and miR-210 significantly promoted the expression of vascular edothelial growth factor (VEGF) in BMSCs in a time-dependent manner (p < 0.05). And miR-210 suppressed PPAR. expression but increased the expression of ALP and osterix, demonstrating that miR-210 inhibited adipocyte differentiation and promoted osteoblast differentiation of BMSCs in vitro. The protein expression of hypoxia-inducible factor 1 alpha (HIF-1 alpha) and VEGF in 17 beta-estradiol (E2) treated osteoblasts were significantly increased in a dose- and time-dependent manner (p < 0.05). And E2 inducted the VEGF expression through the PI3K/AKT signaling pathway in osteoblasts. Taken together, these data implied that miR-210 played an important role in ameliorating the estrogen deficiency caused-postmenopausal osteoporosis through promotion the VEGF expression and osteoblast differentiation.

• 出版日期2015-4
• 单位同济大学