Activation of IFN pathways and plasmacytoid dendritic cell recruitment in target organs of primary Sjogren's syndrome

作者：Gottenberg JE; Cagnard N; Lucchesi C; Letourneur F; Mistou S; Lazure T; Jacques S; Ba N; Ittah M; Lepajolec C; Labetoulle M; Ardizzone M; Sibilia J; Fournier C; Chiocchia G; Mariette X^*

来源：Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(8): 2770-2775.

DOI：10.1073/pnas.0510837103

• autoimmune disease
• innate immunity
• salivary gland

Gene expression analysis of target organs might help provide new insights into the pathogenesis of autoimmune diseases. We used global gene expression profiling of minor salivary glands to identify patterns of gene expression in patients with primary Sjogren's syndrome (pSS), a common and prototypic systemic autoimmune disease. Gene expression analysis allowed for differentiating most patients with pSS from controls. The expression of 23 genes in the IFN pathways, including two Toll-like receptors (TLR8 and TLR9), was significantly different between patients and controls. Furthermore, the increased expression of IFNI-inducible genes, BAFF and IFIN-induced transmembrane protein 1, was also demonstrated in ocular epithelial cells by quantitative RT-PCR. In vitro activation showed that these genes were effectively modulated by IFNs in salivary gland epithelial cells, the target cells of autoimmunity in pSS. The activation of IFN pathways led us to investigate whether plasmacytoid dendritic cells were recruited in salivary glands. These IFN-producing cells were detected by immunohistochemistry in all patients with pSS, whereas none was observed in controls. In conclusion, our results support the pathogenic interaction between the innate and adaptive immune system in pSS. The persistence of the IFN signature might be related to a vicious circle, in which the environment interacts with genetic factors to drive the stimulation of salivary TLRs.

• 出版日期2006-2-21