Promising anticancer activity of a lichen, Parmelia sulcata Taylor, against breast cancer cell lines and genotoxic effect on human lymphocytes

作者：Ari Ferda^*; Ulukaya Engin; Oran Seyhan; Celikler Serap; Ozturk Sule; Ozel Mustafa Zafer

来源：Cytotechnology, 2015, 67(3): 531-543.

DOI：10.1007/s10616-014-9713-4

• Parmelia sulcata
• Cell death
• Apoptosis
• Breast cancer
• Treatment
• DNA damage

Plants are still to be explored for new anticancer compounds because overall success in cancer treatment is still not satisfactory. As a new possible source for such compounds, the lichens are recently taking a great attention. We, therefore, explored both the genotoxic and anti-growth properties of lichen species Parmelia sulcata Taylor. The chemical composition of P. sulcata was analyzed with comprehensive gas chromatography-time of flight mass spectrometry. Anti-growth effect was tested in human breast cancer cell lines (MCF-7 and MDA-MB-231) by the MTT and ATP viability assays, while the genotoxic activity was studied by assays for micronucleus, chromosomal aberration and DNA fragmentation in human lymphocytes culture. Cell death modes (apoptosis/necrosis) were morphologically assessed. P. sulcata inhibited the growth in a dose-dependent manner up to a dose of 100 mu g/ml and induced caspase-independent apoptosis. It also showed genotoxic activity at doses (>125 mu g/ml) higher than that required for apoptosis. These results suggest that P. sulcata may induce caspase-independent apoptotic cell death at lower doses, while it may be genotoxic at relatively higher doses.

• 出版日期2015-5