Au/Fe3O4 core-shell nanoparticles are an efficient immunochromatography test strip performance enhancera comparative study with Au and Fe3O4 nanoparticles

作者：Huang, Jiaoling; Xie, Zhixun; Xie, Liji; Xie, Zhiqin; Luo, Sisi; Deng, Xianwen; Huang, Li; Zeng, Tingting; Zhang, Yanfang; Wang, Sheng; Zhang, Minxiu

来源：RSC Advances, 2018, 8(25): 14064-14071.

DOI：10.1039/c8ra00185e

Immunochromatography test strips that use metal particles constructed from Au, Fe3O4, and Au/Fe3O4 nanoparticles were developed for the rapid detection of avian influenza virus subtype H7 (AIV H7). The principle of this immunochromatography test strip was based on a sandwich immunoreaction in which AIV H7 antigens bind specifically to their corresponding antibodies on a nitrocellulose membrane. An antibody-metal (Au, Fe3O4 or Au/Fe3O4) nanoparticle conjugate was used as a label and coated onto a glass fiber membrane, which was used as a conjugate pad. To create a test and a control zone, an anti-H7 polyclonal antibody and an anti-IgG antibody were immobilized onto the nitrocellulose membrane, respectively. Positive samples displayed brown/red lines in the test and control zones of the nitrocellulose membrane, whereas negative samples resulted in a brown/red line only in the control zone. The limit of detection (LOD) of the Au/Fe3O4 nanoparticle-based immunochromatography test strips was found to be 10(3.5) EID50 (EID50: 50% Egg Infective Dose), which could be visually detected by the naked eye within 15 min. In addition, 200 clinical samples were tested using the Au/Fe3O4 nanoparticle-based immunochromatography test strip to estimate its performance, and seven were positive for AIV H7. In summary, the Au/Fe3O4 nanoparticle-based immunochromatography test strip offers a simple and cost-effective tool for the rapid detection of AIV H7.

• 出版日期2018
• 单位广西壮族自治区兽医研究所