New Strategy for High-Level Expression and Purification of Biologically Active Monomeric TGF-beta 1/C77S in Escherichia coli

作者：Kim Yana V; Gasparian Marine E^*; Bocharov Eduard V; Chertkova Rita V; Tkach Elena N; Dolgikh Dmitry A; Kirpichnikov Mikhail P

来源：Molecular Biotechnology, 2015, 57(2): 160-171.

DOI：10.1007/s12033-014-9812-7

• TGF-beta 1
• Monomeric TGF-beta 1
• TGF-beta 1/C77S
• Thioredoxin-tag fusion protein
• Escherichia coli
• Refolding
• CD spectrum

Mature transforming growth factor beta1 (TGF-beta 1) is a homodimeric protein with a single disulfide bridge between Cys77 on the respective monomers. The synthetic DNA sequence encoding the mature human TGF-beta 1/C77S (further termed TGF-beta 1m) was cloned into plasmid pET-32a downstream to the gene of fusion partner thioredoxin (Trx) immediately after the DNA sequence encoding enteropeptidase recognition site. High-level expression (similar to 1.5 g l(-1)) of Trx/TGF-beta 1m fusion was achieved in Escherichia coli BL21(DE3) strain mainly in insoluble form. The fusion was solubilized and refolded in glutathione redox system in the presence of zwitterionic detergent CHAPS. After refolding, Trx/TGF-beta 1m fusion was cleaved by enteropeptidase, and the carrier protein of TGF-beta 1m was separated from thioredoxin on Ni-NTA agarose. Separation of monomeric molecules from the noncovalently bounded oligomers was done using cation-exchange chromatography. The structure of purified TGF-beta 1m was confirmed by circular dichroism analysis. The developed technology allowed purifying biologically active tag-free monomeric TGF-beta 1m from bacteria with a yield of about 2.8 mg from 100 ml cell culture. The low-cost and easy purification steps allow considering that our proposed preparation of recombinant monomeric TGF-beta 1 could be employed for in vitro and in vivo experiments as well as for therapeutic intervention.

• 出版日期2015-2