An antibody raised against a pathogenic serpin variant induces mutant-like behaviour in the wild-type protein

作者：Irving James A^*; Miranda Elena; Haq Imran; Perez Juan; Kotov Vadim R; Faull Sarah V; Motamedi Shad Neda; Lomas David A

来源：Biochemical Journal, 2015, 468(1): 99-108.

DOI：10.1042/BJ20141569

• aggregation
• antibody
• alpha(1)-antitrypsin
• polymerization
• potein stability

A monoclonal antibody (mAb) that binds to a transient intermediate may act as a catalyst for the corresponding reaction; here we show this principle can extend on a macro molecular scale to the induction of mutant-like oligomerization in a wild-type protein. Using the common pathogenic E342K (Z) variant of a1-antitrypsin as antigen - whose native state is susceptible to the formation of a proto-oligomeric intermediate - we have produced a mAb (5E3) that increases the rate of oligomerization of the wild-type (M) variant. Employing ELISA, gel shift, thermal stability and FRETtime-course experiments, we showthatmAb(5E3) does not bind to the native state of alpha(1)-antitrypsin, but recognizes a cryptic epitope in the vicinity of the post-helix A loop and strand 4C that is revealed upon transition to the polymerization intermediate, and which persists in the ensuing oligomer. This epitope is not shared by loop-inserted monomeric conformations. We show the increased amenity to polymerization by either the pathogenic E342K mutation or the binding of mAb(5E3) occurs without affecting the energetic barrier to polymerization. As mAb(5E3) also does not alter the relative stability of the monomer to intermediate, it acts in a manner similar to the E342K mutant, by facilitating the conformational interchange between these two states.

• 出版日期2015-5-15
• 单位河北医科大学