Analysis of cyclic pepticies in Pseudostellaria heterophylla (Miq.) Pax by HPLC-APCI-MS

作者：Shen Yan^*; Han Chao; Chen Junhui; Wan Xiaoru

来源：Chromatographia, 2007, 66(5-6): 319-323.

DOI：10.1365/s10337-007-0333-4

• column liquid chromatography
• HPLC-APCI-MS
• Traditional Chinese Medicine
• cyclic peptides
• Pseudostellaria heterophylla (Miq.) Pax

A new method for the analysis of cyclic peptides (Pseudostellarins) in traditional Chinese medicine, Pseudostellaria heterophylla (Miq.) Pax, was established by high performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS). The real samples were separated by a reversed-phase C-18 column using a binary eluent under gradient conditions. Six cyclic peptides were isolated and detected with a DAD detector. The Pseudostellarins components were identified by APCI-MS in a negative mode. in the negative ion APCI mode, the best sensitivity and the lowest detection limit for Pseudostellarins were obtained by using a mobile phase consisting of acetonitrile and pure water. The APCI spectra were characterized by [M-H](-) peak. The parent ions [M-H](-) with highest intensity was used for quantification of one cyclic peptide-Pseudostellarin B.

• 出版日期2007-9
• 单位国家海洋局第一海洋研究所; 温州大学