Accumulation of gamma-aminobutyric acid in germinated soybean (Glycine max L.) in relation to glutamate decarboxylase and diamine oxidase activity induced by additives under hypoxia

作者：Guo, Yuanxin; Yang, Runqiang; Chen, Hui; Song, Yu; Gu, Zhenxin^*

来源：European Food Research and Technology, 2012, 234(4): 679-687.

DOI：10.1007/s00217-012-1678-y

• GABA accumulation
• Germinated soybean
• Glutamate decarboxylase (GAD)
• Diamine oxidase activity (DAO)
• Hypoxia stress

High levels of gamma-aminobutyric acid (GABA) accumulate in plant tissues under various stresses and exogenous additives. The purpose of this research is to provide an effective finding that can prove a rapid accumulation of GABA in germinated soybean (Glycine max L.) in response to different additives under hypoxia. Hypoxia-induced GABA accumulation in soybean embryo resulted in part from polyamine oxidation. Response to different concentration of glutamate (Glu), pyridoxal phosphate, arginine, CuCl2, NaCl, and CaCl2, a significant difference including GABA accumulation, changes of Glutamate decarboxylase (GAD), and Diamine oxidase activity (DAO) activity in germinated soybean under hypoxia occurred (p < 0.05) and the maximum accumulation of GABA were 4.07, 3.02, 3.50, 3.26, 4.00, and 3.30 g kg(-1) DW respectively, which were significantly higher than those germinated soybean under normal culture (CK) and hypoxia culture (CK0) (p < 0.05). The GAD and DAO have different distributions in cotyledon and embryo of germinated soybean, and the enzyme activity mainly located in embryo of germinated soybean. Germinated soybean is a good resource of GABA-rich food. Different additives have significant effects on GABA production, among which Glu and NaCl are ideal material for GABA accumulation.

• 出版日期2012-4
• 单位南京农业大学; 安徽科技学院