Novosphingobium chloroacetimidivorans sp nova, a chloroacetamide herbicide-degrading bacterium isolated from activated sludge

作者：Chen, Qing; Zhang, Jun; Wang, Cheng-Hong; Jiang, Jin; Kwon, Soon-Wo; Sun, Li-Na; Shen, Wen-Biao; He, Jian^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2014, 64(Pt 8): 2573-2578.

DOI：10.1099/ijs.0.062950-0

• MAXIMUM-LIKELIHOOD
• LIPID-COMPOSITION
• BIODEGRADATION
• DEGRADATION
• STRAINS

Strain BUT-14(T), a Gram-reaction-negative, non-spore-forming, ellipse-shaped bacterium, was isolated from activated sludge of a chloroacetamide-herbicides-manufacturing wastewater treatment facility. The strain was able to degrade more than 90% of butachlor, acetochlor and alachlor (100 mg l(-1)) within 5 days of incubation. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BUT-14(T) was a member of the genus Novosphingobium and showed the highest sequence similarities to Novosphingobium soli DSM 22821(T) (97.9%), N. naphthalenivorans KACC 15258(T) (97.4%), N. pentaromativorans JCM 12182(T) (97.4%) and N. barchaimii DSM 25411(T) (97.1 CYO and lower (<97%) sequence similarities to all other species of the genus Novosphingobium. Chemotaxonomic analysis revealed that strain BUT-14(T) possessed Q-10 as the predominant ubiquinone, spermidine as the major polyamine and C-18:1 omega 7c (46.9%), C-17:0 omega 6c (17.9%), summed feature 3, C-14:0 2-OH (4.4%), C-15:0 2-OH (3.1%) and C-16:0 (5.51%) as the major fatty acids. The polar lipids included lipid, glycolipid, phosphatidylglycerol, phospholipid, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and phospatidyldimethylethanolamine. Strain BUT-14(T) showed low DNA DNA relatedness with N. soli DSM 22821(T) (41.5 +/- 2.9 %), N. naphthalenivorans JCM 12182(T) (49.2 +/- 4.2 %), N. pentaromativorans KACC 12295(T) (53.2 1.9%) and N. barchaimii DSM 2541(T) (51.2 +/- 4.5 %). The DNA G+C content was 66 +/- 0.3 molok. The combination of phylogenetic analysis, phenotypic characteristics, chemotaxonomic data and DNA DNA hybridization supports the suggestion that strain BUT-14(T) represents a novel species of the genus Novosphingobium, for which the name Novosphingobium chloroacetimidivorans sp. nov. is proposed. The type strain is BUT-14(T) (=CCTCC AB 2013086(T)=KACC 17147(T)=JCM 19923(T)).

• 出版日期2014-8
• 单位南京农业大学