A metabolomics and proteomics study of the adaptation of Staphylococcus aureus to glucose starvation

作者：Liebeke Manuel; Doerries Kirsten; Zuehlke Daniela; Bernhardt Joerg; Fuchs Stephan; Pane Farre Jan; Engelmann Susanne; Voelker Uwe; Bode Ruediger; Dandekar Thomas; Lindequist Ulrike; Hecker Michael; Lalk Michael^*

来源：Molecular Biosystems, 2011, 7(4): 1241-1253.

DOI：10.1039/c0mb00315h

As a versatile pathogen Staphylococcus aureus can cause various disease patterns, which are influenced by strain specific virulence factor repertoires but also by S. aureus physiological adaptation capacity. Here, we present metabolomic descriptions of S. aureus central metabolic pathways and demonstrate the potential for combined metabolomics- and proteomics-based approaches for the basic research of this important pathogen. This study provides a time-resolved picture of more than 500 proteins and 94 metabolites during the transition from exponential growth to glucose starvation. Under glucose excess, cells exhibited higher levels of proteins involved in glycolysis and protein-synthesis, whereas entry into the stationary phase triggered an increase of enzymes of TCC and gluconeogenesis. These alterations in levels of metabolic enzymes were paralleled by more pronounced changes in the concentrations of associated metabolites, in particular, intermediates of the glycolysis and several amino acids.

• 出版日期2011