Anti-Inflammatory Effects of Cumin Essential Oil by Blocking JNK, ERK, and NF-kappa B Signaling Pathways in LPS-Stimulated RAW 264.7 Cells

作者：Wei, Juan; Zhang, Xitong; Bi, Yang^*; Miao, Ruidong; Zhang, Zhong; Su, Hailan

来源：Evidence-Based Complementary and Alternative Medicine, 2015, 2015: 474509.

DOI：10.1155/2015/474509

• NITRIC-OXIDE SYNTHASE
• CHEMICAL-COMPOSITIONS
• CYMINUM
• CYCLOOXYGENASE-2
• MACROPHAGES
• MAPK
• INFLAMMATION
• NO

Cumin seeds (Cuminum cyminum L.) have been commonly used in food flavoring and perfumery. In this study, cumin essential oil (CuEO) extracted from seeds was employed to investigate the anti-inflammatory effects in lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells and the underlying mechanisms. A total of 26 volatile constituents were identified in CuEO by GC-MS, and the most abundant constituent was cuminaldehyde (48.773%). Mitochondrial-respiration-dependent 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium (MTT) reduction assay demonstrated that CuEO did not exhibit any cytotoxic effect at the employed concentrations (0.0005-0.01%). Real-time PCR tests showed that CuEO significantly inhibited the mRNA expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), interleukin- (IL-) 1, and IL-6. Moreover, western blotting analysis revealed that CuEO blocked LPS-induced transcriptional activation of nuclear factor-kappa B (NF-kappa B) and inhibited the phosphorylation of extracellular signal regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). These results suggested that CuEO exerted anti-inflammatory effects in LPS-stimulated RAW 264.7 cells via inhibition of NF-kappa B and mitogen-activated protein kinases ERK and JNK signaling; the chemical could be used as a source of anti-inflammatory agents as well as dietary complement for health promotion.

• 出版日期2015
• 单位甘肃农业大学; 兰州大学