Antifungal susceptibilities and identification of species of the Sporothrix schenckii complex isolated in Brazil

作者：Ottonelli Stopiglia Cheila Denise; Magagnin Cibele Massotti; Castrillon Mauricio Ramirez; Camargo Mendes Sandra Denise; Heidrich Daiane; Valente Patricia; Scroferneker Maria Lucia^*

来源：Medical Mycology, 2014, 52(1): 56-64.

DOI：10.3109/13693786.2013.818726

• Sporothrix
• sporotrichosis
• antifungal agents

Sporotrichosis is a subacute or chronic mycosis caused worldwide by the dimorphic species complex, Sporothrix schenckii. We studied 85 isolates recovered in Brazil to verify their identification and evaluate their in vitro antifungal susceptibility patterns. Based on phenotypic tests (microscopic features, ability to grow at 30 degrees C and 37 degrees C, colony diameters, as well as assimilation of sucrose and raffinose) and molecular assays (amplification of a fragment of the calmodulin gene), the strains were identified as S. schenckii, S. brasiliensis and S. globosa, with a predominance of S. schenckii isolates. There was 37.7% disagreement between the phenotypic and genotypic identification methodologies. In general, terbinafine was the most active drug, followed by ketoconazole and itraconazole, and the less active fluconazole and voriconazole. Five isolates (one S. globosa and four S. schenckii) were found to be itraconazole-resistant strains but, in general, there were no differences in the in vitro antifungal susceptibility profiles among the Sporothrix species.

• 出版日期2014-1