Astragaloside IV ameliorates allergic inflammation by inhibiting key initiating factors in the initial stage of sensitization

作者：Bao, Kai-fan; Yu, Xi; Wei, Xiao; Gui, Li-li; Liu, Hai-liang; Wang, Xiao-yu; Tao, Yu; Jiang, Guo-rong^*; Hong, Min^*

来源：Scientific Reports, 2016, 6(1): 38241.

DOI：10.1038/srep38241

• THYMIC STROMAL LYMPHOPOIETIN
• INNATE LYMPHOID-CELLS
• ATOPIC-DERMATITIS
• CONTACT-DERMATITIS
• EPITHELIAL-CELLS
• ASTHMA
• TSLP
• IMMUNITY
• DISEASE
• IL-33

To illuminate the anti-allergy mechanism of astragaloside IV (AS-IV), we assessed its effects in a murine model of allergic contact dermatitis (ACD). AS-IV administered in the sensitization phase, rather than in the elicitation phase, dramatically alleviated the symptoms of allergic inflammation. We hypothesized that AS-IV exerts its anti-allergy effects by regulating the production of key pro-allergic cytokines based on the fact that interleukin (IL)-33 and thymic stromal lymphopoietin (TSLP) levels increase significantly in the initial stage of the sensitization phase. AS-IV administered in the initial stage of ACD inhibited TSLP and IL-33 expression and reduced the proportion of type-2 innate lymphoid cells (ILC2s). An in vitro study showed that the production of pro-allergic cytokines was significantly inhibited in AS-IV presenting HaCaT cells. We also verified that AS-IV administered only in the initial stage markedly alleviated inflammation, including ear swelling, Th2 cytokine expression, and histological changes. Taken together, these results suggest that AS-IV effectively ameliorates the progression of allergic inflammation by inhibiting key initiating factors, including TSLP and IL-33, and can be used to prevent and/or treat patients with ACD. Our data also suggest that these key pro-allergic cytokines are potential therapeutic targets for allergic diseases.

• 出版日期2016-12-5
• 单位南京中医药大学; 苏州市中医医院