A new strategy for photoelectrochemical DNA biosensor using chemiluminescence reaction as light source

作者：Zhang, Xiaoru^*; Zhao, Yanqing; Zhou, Huanran; Qu, Bin

来源：Biosensors and Bioelectronics, 2011, 26(5): 2737-2741.

DOI：10.1016/j.bios.2010.09.051

• Photoelectrochemistry
• Chemiluminescence
• DNA biosensor
• Ru(bpY)(2)dppz(2+)

Physical light source is absolutely necessary for usual photoelectrochemical measurement. In this work, chemiluminescence reaction rather than physical light source was used for the development of a novel photoelectrochemical DNA biosensor. CIPO (bis(2,4,5-trichlro-6-n-pentoxycarbonylphenyl)oxalate)-H2O2-9,10-diPhenylanthrancene was selected as a CL system, which can produce appropriate exciting light and excite photoelectro active materials Ru(bpy)(2)dppz(2+) intercalated into the double-stranded DNA. Using such simple intercalation method, a detection limit of 4.5 x 10(-9) M target DNA was achieved without any amplification process. In addition, the selected CL system could be used to excite AuNPs-Ru(bpy)(2)dppz(2+) complex as well as CdSe QD multilayer, which indicated a good applicability for the established method.

• 出版日期2011-1-15
• 单位青岛科技大学