An extracellular protease with depilation activity from Streptomyces nogalator

作者：Mitra P; Chakrabartty PK^*

来源：Journal of Scientific & Industrial Research, 2005, 64(12): 978-983.

• depilation
• enzyme purification
• proteolytic enzyme
• Streptomyces nogalator

An extracellular protease-producing actinomycete, Streptomyces nogalator strain Ac 80, was isolated from soil. Mutants of the strain designated as Mt C producing protease of higher (3.0 mg tyrosine liberated/min/mg protein) and Mt L of lower (0.9 mg tyrosine liberated/min/mg protein) specific activity as compared to that of the wild type (1.8 mg tyrosine liberated/min/mg protein) were selected. Proteolytic activity of the wild type and the mutants correlated with their depilation capacities. Proteolytic enzyme produced by Mt C in solid state fermentation was purified to homogeneity, and partially characterized (molecular mass, 66 kDa by SDS- PAGE). It was found to be efficient in depilation of goatskin.

• 出版日期2005-12
• 单位常州工学院