Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2

作者：Ye, Guang-yong^*; Wang, Ke-yi; Gui, Qiao-di; Wang, Min

来源：Journal of Zhejiang University-Science B(Biomedicine & Biotechnology), 2018, 19(8): 654-661.

DOI：10.1631/jzus.B1800005

• Ureaplasma urealyticum
• Lipid-associated membrane protein
• Human amniotic epithelial cell
• Toll-like receptor 2

The purpose of this study was to determine the role of Ureaplasma urealyticum-derived lipidassociated membrane proteins (LAMPs) in the host innate immune system, specifically their effect on Toll-like receptors (TLRs). @@@ LAMPs were derived from U. urealyticum strains, and human amniotic epithelial cells (HAECs) were isolated from healthy full-term placentas. Cytokine concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and TLR2 mRNA by real-time PCR. Expression of TLR2 was confirmed by Western blotting and immunohistochemistry. @@@ LAMPs induced HAECs to produce inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-alpha. Cytokine production was reduced after blocking TLR2 using TLR2 inhibitor (anti-hTLR2-IgA). @@@ LAMPs isolated from U. urealyticum induced TLR2-dependent up-regulation of inflammatory genes and cytokines in HAECs.

• 出版日期2018-8
• 单位浙江大学; 杭州市第一人民医院; 陕西省人民医院; 西安医学院