A striking local esophageal cytokine expression profile in eosinophilic esophagitis

作者：Blanchard Carine; Stucke Emily M; Rodriguez Jimenez Beatriz; Burwinkel Karen; Collins Margaret H; Ahrens Annette; Alexander Eileen S; Buckmeier Bridget K; Jameson Sean C; Kaul Ajay; Franciosi James P; Kushner Jonathan P; Putnam Philip E; Abonia J Pablo; Rothenberg Marc E^*

来源：Journal of Allergy and Clinical Immunology, 2011, 127(1): 208-U341.

DOI：10.1016/j.jaci.2010.10.039

• Eosinophilic esophagitis
• eosinophils
• cytokines
• biomarkers
• eotaxin-3/CCL26
• allergy

Background: Eosinophilic esophagitis (EE) is an emerging worldwide disease that mimics gastroesophageal reflux disease.
Objective: Early studies have suggested that esophageal eosinophilia occurs in association with T(H)2 allergic responses, yet the local and systemic expression of relevant cytokines has not been well characterized.
Methods: A human inflammatory cytokine and receptor PCR array containing 84 genes followed by PCR validation and multiplex arrays were used to quantify cytokine mRNA in esophageal biopsies and blood samples.
Results: Esophageal transcripts of numerous chemokines (eg, chemokine [C-C motif] ligand [CCL] 1, CCL1, CCL23, CCL26 [eotaxin-3], chemokine [C-X-C motif] ligand [CXCL] 1, and CXCL2), cytokines (eg, IL13 and ATP-binding cassette, subfamily F, member 1), and cytokine receptors (eg, IL5 receptor, alpha) were induced at least 4-fold in individuals with EE. Analysis of esophageal biopsies (n = 288) revealed that eotaxin-3 mRNA level alone had 89% sensitivity for distinguishing individuals with and without EE. The presence of allergy was associated with significantly increased esophageal expression of IL4 and IL5 mRNA in patients with active EE. We identified 8 cytokines (IL-4, IL-13, IL-5, IL-6, IL-12p70, CD40 ligand, IL-1 alpha, and IL-17) whose blood levels retrospectively distinguished 12 patients without EE from 13 patients with EE with 100% specificity and 100% sensitivity. When applied to a blind, prospectively recruited group of 36 patients, the cytokine panel scoring system had a 79% positive predictive value, 68% negative predictive value, 61% sensitivity, and 83% specificity for identifying EE.
Conclusion: Evidence is presented that IL13 and IL5 associate with eosinophil and eotaxin-3 levels, indicating the key role of adaptive T(H)2 immunity in regulating eotaxin-3-driven esophageal eosinophilia in the absence of a consistent systemic change in cytokines. (J Allergy Clin Immunol 2011;127:208-17.)

• 出版日期2011-1