An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro

作者：Tougan Takahiro; Suzuki Yuhgi; Itagaki Sawako; Izuka Munehisa; Toya Yuji; Uchihashi Kinya; Horii Toshihiro^*

来源：Malaria Journal, 2018, 17(1): 59.

DOI：10.1186/s12936-018-2208-6

• Plasmodium falciparum
• in vitro culture
• Blood stage
• Flow cytometry
• XN-30 analyzer

Background: The automated haematology analyzer XN-30 (Sysmex, Kobe, Japan) easily and rapidly detects malarial parasites in clinical blood samples using flow cytometry. The XN-30 analyzer is able to distinguish each developmental stage by measuring DNA content and cell size. Thus, it was expected to be capable of quantifying the developmental stages of cultured falciparum parasite. To achieve this requirement, a modified algorithm was tested for its validity and reliability using in vitro cultured falciparum parasite.
Results: The XN-30 analyzer automatically measured each developmental stage as well as total parasitaemia. Comparison of the parasitaemia obtained using the XN-30 analyzer equipped with the modified algorithm with that obtained using microscopy examination of Giemsa-stained smears revealed the greater sensitivity and reproducibility of the former. The XN-30 analyzer also detected free merozoites and purified gametocytes.
Conclusions: The XN-30 analyzer allows the precise recognition and enumeration of total and each developmental stages of cultured falciparum parasites, and permits the sensitive and reproducible calculation of parasitaemia. The results indicate the potential of the XN-30 analyzer for basic research on malarial biology, anti-malarial drug discovery, and evaluation of drug efficacy.

• 出版日期2018-2-2