Acetylcholinesterase activity, antioxidant defenses, and lipid peroxidation in the clam Semele solida: Can this species be used as a bioindicator?

作者：Srain Benjamin^*; Rudolph Anny

来源：Revista de Biologia Marina y Oceanografia, 2010, 45(2): 227-233.

• Biomarkers
• bioindicadores
• marine bivalves
• programs of environmental alertness

We analyzed the relationships between biochemical parameters: acetylcholinesterase activity (AChE); glutathione S-transferase activity (GST); glutathione concentration (GSH) and lipid peroxidation; in the bivalve mollusc Semele solida, with the effects of environmental stress (anthropogenic activity), from three estuaries in Eastern South Pacific bays: Coliumo estuary (in Coliumo Bay), Lenga estuary (in San Vicente Bay) and Andalien estuary (in Concepcion Bay). Coliumo is the estuary with minor environmental stress. Thirty juveniles from each site were selected for individual analyses in homogenized of gill or digestive gland tissue to assess AChE activity, GST activity, GSH concentration, malonyldialdehyde concentration (MDA) (lipid peroxidation), and protein content. The biochemical parameters analyzed in specimens from Coliumo estuary, differed significantly from that in Andalien estuary, with lower AChE activity in gill tissue (2189.9 +/- 189.6 mu mol min(-1) mg protein(-1)) and intracellular GSH levels (59.8 +/- 13.3 mu M), and the highest lipid peroxidation (31.9 +/- 7.4 nmol MDA mL(-1)) and GST activity (614.9 +/- 92.3 mu mol min(-1) mg protein(-1)). The biochemical parameters in S. solida were closely related to the sector's stress. Due to this bivalve sensitivity, is recommended as bioindicator for use in programs of environmental alertness in the Eastern South Pacific coastal zone.

• 出版日期2010