Application of pseudo-two phase partitioning bioreactor (P-TPPB) to the production of biodiesel

作者：Go Young Wook; Yeom Sung Ho^*

来源：Bioprocess and Biosystems Engineering, 2014, 37(2): 269-275.

DOI：10.1007/s00449-013-0992-5

• Pseudo-two phase partitioning bioreactor
• Enzyme
• Biodiesel
• Methanol
• Inhibition

Pseudo-two phase partitioning bioreactor (P-TPPB) was newly proposed as an extension of the application of TPPB to bioprocesses in which hydrophilic substrates and/or products are involved. The feasibility of P-TPPB was demonstrated in enzymatic biodiesel production, where methanol completely inhibits the enzymes. Unlike conventional TPPB, the P-TPPB comprises a hydrophobic first phase (soybean oil) and hydrophilic second phase. n-Pentanol was found to be the optimum for the second phase, since P-TPPB containing n-pentanol showed the greatest total biodiesel conversion and highest fatty acid methyl ester content. The enzyme was repeatedly used to produce biodiesel in P-TPPB, while maintaining its activity at over 95 % relative to that of the intact enzyme.

• 出版日期2014-2