Up-regulation of GABA transporters and GABAA receptor α1 subunit in tremor rat hippocampus

作者：Mao, Xiaoyuan; Guo, Feng; Yu, Junling; Min, Dongyu; Wang, Zhanyou; Xie, Ni; Chen, Tianbao; Shaw, Chris; Cai, Jiqun^*

来源：Neuroscience Letters, 2010, 486(3): 150-155.

DOI：10.1016/j.neulet.2010.09.033

• Tremor rat
• GABA
• GABA transporters
• Hippocampus
• Synaptosome
• GABA receptor

The loss of GABAergic neurotransmission has been closely linked with epileptogenesis The modulation of the synaptic activity occurs both via the removal of GABA from the synaptic cleft and by GABA transporters (GATs) and by modulation of GABA receptors The tremor rat (TRM tm/tm) is the parent strain of the spontaneously epileptic rat (SER tm/tm) which exhibits absence-like seizure after 8 weeks of age However there are no reports that can elucidate the effects of GATs and GABA(A) receptors (GABARs) on TRMs The present study was conducted to detect GATs and GABAR alpha 1 subunit in TRMs hippocampus at mRNA and protein levels In this study total synaptosomal GABA content was significantly decreased in TRMs hippocampus compared with control Wistar rats by high performance liquid chromatography (HPLC) mRNA and protein expressions of GAT-1 GAT-3 and GABAR alpha 1 subunit were all significantly increased in TRMs hippocampus by real time PCR and Western blot respectively GAT-1 and GABAR alpha 1 subunit proteins were localized widely in TRMs and control rats hippocampus including CA1 CA3 and dentate gyrus (DG) regions whereas only a wide distribution of GAT-3 was observed in CA1 region by immunohistochemistry These data demonstrate that exc

• 出版日期2010-12-17
• 单位中国医科大学