Antifungal Activity and Isomerization of Octadecyl p-coumarates from Ipomoea carnea subsp fistulosa

作者：Nidiry Eugene Sebastian J^*; Ganeshan Girija; Lokesha Ankanahalli N

来源：Natural Product Communications, 2011, 6(12): 1889-1892.

• Ipomoea carnea
• Ipomoea fistulosa
• Convulvulaceae
• antifungal
• (E) octadecyl p-coumarate
• (Z) octadecyl p-coumarate
• isomerization
• 2D NMR techniques

Bioassay monitored HPLC assisted isolation and purification of the chief antifungal fraction of the leaves of Ipomoea cornea subsp. fistulosa (Convulvulaceae) were achieved using Colletotrichum gloeosporioides and Cladosporium cucumerinum as test organisms. The activity of the purified fraction was further confirmed by the dose dependent inhibition of the spore germination of Alternaria alternata and A. porri. The active fraction was identified as a mixture of (E)-octadecyl p-coumarate and (Z)-octadecyl p-coumarate. The two isomers were detected on an HPLC column with substantially different retention times, but once eluted from the column, one form was partly converted to the other in daylight. Conclusive evidence for the structures and their isomerization were obtained from the HPLC behavior, IR, UV, HRESIMS, CIMS and NMR spectral data. Important (1)H NMR and (13)C NMR signals could be separately assigned for the isomers using 2D NMR techniques.

• 出版日期2011-12