Different MicroRNA Expression Levels in Gastric Cancer Depending on Helicobacter pylori Infection

作者：Chang Hyun; Kim Nayoung^*; Park Ji Hyun; Nam Ryoung Hee; Choi Yoon Jeong; Lee Hye Seung; Yoon Hyuk; Shin Cheol Min; Park Young Soo; Kim Jung Min; Lee Dong Ho

来源：Gut and Liver, 2015, 9(2): 188-196.

DOI：10.5009/gnl13371

• Gastric carcinogenesis
• Helicobacter pylori
• Microarray
• MicroRNAs
• TaqMan miRNA assays

Background/Aims: This study was conducted to identify microRNAs (miRNAs) that are differentially expressed in Helicobacter pylori-infected patients with an intestinal type of gastric cancer using miRNA microarray and to confirm the candidate miRNA expression levels. Methods: Total RNA was extracted from the cancerous and noncancerous regions of formalin-fixed, paraffin-embedded tissues of Helicobacter pylori-positive (n=8) or Helicobacter pylori-negative (n=8) patients with an intestinal type of gastric cancer. RNA expression was analyzed using a 3,523.miRNA profiling microarray based on the Sanger miRBase. Validation analysis was performed using TaqMan miRNA assays. Results: A total of 219 miRNAs in the aberrant miRNA profiles across the miRNA microarray showed at least a 2-fold change differential expression in Helicobacter pylori-positive and Helicobacter pylori-negative cancer tissues. After candidate miRNAs were selected using online miRNA databases, TaqMan miRNA assays confirmed that three miRNAs (miR-99b-3p, miR-564, and miR-638) were significantly increased in three Helicobacter pylori-positive cancer tissues compared to the Helicobacter pylori-negative cancer tissue. Additionally, four miRNAs (miR-204-5p, miR-338-5p, miR-375, and miR-548c-3p) were significantly increased in Helicobacter pylori-negative cancer tissues compared to Helicobacter pylori-positive cancer tissues. Conclusions: miRNA expression in the intestinal type of Helicobacter pylori infection-dependent gastric cancer suggests that different gastric cancer pathogenesis mechanisms could exist between Helicobacter pylori-positive and Helicobacter pylori-negative gastric cancer. Additional functional studies are required.

• 出版日期2015-3