Arabidopsis transcriptome analysis under drought, cold, high-salinity and ABA treatment conditions using a tiling array

作者：Matsui Akihiro; Ishida Junko; Morosawa Taeko; Mochizuki Yoshiki; Kaminuma Eli; Endo Takaho A; Okamoto Masanori; Nambara Eiji; Nakajima Maiko; Kawashima Makiko; Satou Masakazu; Kim Jong Myong; Kobayashi Norio; Toyoda Tetsuro; Shinozaki Kazuo; Seki Motoaki^*

来源：Plant and Cell Physiology, 2008, 49(8): 1135-1149.

DOI：10.1093/pcp/pcn101

• abiotic stress
• antisense RNA
• Arabidopsis
• non-protein-coding RNA
• tiling array

Plants respond and adapt to drought, cold and high-salinity stresses in order to survive. In this study, we applied Arabidopsis Affymetrix tiling arrays to study the whole genome transcriptome under drought, cold, high-salinity and ABA treatment conditions. The bioinformatic analysis using the tiling array data showed that 7,719 non-AGI transcriptional units (TUs) exist in the unannotated intergenic regions of Arabidopsis genome. These include 1,275 and 181 TUs that are induced and downregulated, respectively, by the stress or ABA treatments. Most of the non-AGI TUs are hypothetical non-protein-coding RNAs. About 80 of the non-AGI TUs belong to pairs of the fully overlapping sense-antisense transcripts (fSATs). Significant linear correlation between the expression ratios (treated/untreated) of the sense TUs and the ratios of the antisense TUs was observed in the SATs of AGI code/non-AGI TU. We studied the biogenesis mechanisms of the stress- or ABA-inducible antisense RNAs and found that the expression of sense TUs is necessary for the stress- or ABA-inducible expression of the antisense TUs in the fSATs (AGI code/non-AGI TU).

• 出版日期2008-8