科研之友
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摘要
A method using LC was developed for determination of ochratoxin A (OTA) in feeds.The extracted samples were cleaned up by an immunoaffinity column prepared by covalently coupling polyclonal antibodies against OTA to cyanogen bromide-activated Sepharose 4B. The eluates were determined by LC with fluorescence detection. Recoveries of OTA from fortified samples of 1-10 mu g/kg levels ranged from 84.3 to 90.0%, with CVs of 3.3-7.8%. The detection limit was 0.045 mu g/kg based on an SIN of 3:1.A total of 65 feed samples were screened for OTA with the proposed method. The results showed that only nine samples were contaminated with OTAs at low levels. The presented method was successfully applied to quantify OTAs in real feed samples.
- 出版日期2013-6
