UVA Photoprotective Properties of an Artificial Carotenylflavonoid Hybrid Molecule

作者：Hundsdoerfer Claas; Stahl Wilhelm; Mueller Thomas J J; De Spirt Silke^*

来源：Chemical Research in Toxicology, 2012, 25(8): 1692-1698.

DOI：10.1021/tx300158r

Carotenoids and flavonoids represent two classes of natural antioxidants, a biological activity, which is determined by their chemical structure. To combine their antioxidant properties, a dual functional carotenylflavonoid hybrid molecule was synthesized. The antioxidant activity of this compound was tested in human dermal fibroblasts exposed to UVA irradiation. Test parameters were hemeoxygenase-1 (HO-1) expression, malondialdehyde (MDA), and reactive oxygen species (ROS) formation and cell viability. For comparison, the substructure components of the carotenylflavonoid, 4-hydroxyflavone and 11%26apos;-apo-beta-carotenylbenzene, were also tested. Incubation of cells with the carotenylflavonoid and 11%26apos;-apo-beta-carotenylbenzene attenuated UVA-induced HO-1 expression. In the MDA assay, the carotenylflavonoid and 11%26apos;-apo-beta-carotenylbenzene were moderately effective at low concentrations. At higher concentrations, the compound provoked an increase of MDA, which was confirmed by the H2DCF-DA assay measuring ROS formation. 4-Hydroxyflavone moderately inhibited the formation of MDA at all levels that were tested. The study showed that the carotenylflavonoid counteracts UVA-induced HO-1 expression. However, a photoprotection against lipid oxidation, ROS formation, and cell toxicity could not be proven in the experimental setting.

• 出版日期2012-8