ASSAY OF AMINO ACIDS IN MEDICINAL PLANTS BY TLC (USING STINGING NETTLE LEAVES AND COMMON SEA BUCKTHORN FRUITS AS EXAMPLES)

作者：Trineeva O V^*; Safonova E F; Sinkevich A V; Slivkin A I

来源：Pharmaceutical Chemistry Journal, 2015, 49(5): 323-328.

DOI：10.1007/s11094-015-1278-9

• amino acids
• common sea buckthorn fruits
• stinging nettle leaves
• thin layer chromatography
• capillary electrophoresis

A method for separating and assaying total free amino acids in aqueous extracts of medicinal plants by TLC was developed (using common sea buckthorn fruits and stinging nettle leaves as examples). The amino acid compositions of common sea buckthorn fruits and stinging nettle leaves were determined by capillary electrophoresis. An approach to assaying amino acids in a variety of objects is proposed, based on identification by determination of R (s) values without the use of standards. As the set of amino acids is individual for each type of plant material and depends on the means used for conservation, the chromatographic profile of amino acids can be used for standardization of materials.

• 出版日期2015-8